Faculty
Schulich School of Medicine & Dentistry
Supervisor Name
Dr. Rithwik Ramachandran
Keywords
Pancreas, Cancer, Immunohistochemistry, Immune Checkpoint Inhibitors
Description
My research project aims to examine the expression landscape of the immune checkpoint protein, PD-L1, in pancreatic ductal adenocarcinoma (PDAC) and pancreatitis using automated image analysis tools such as QuPath. Immunohistochemical analysis and confocal microscopy are utilized to identify unique expression patterns, including membrane staining and necrotic areas, compared to those identified in the PD-L1 IHC 22C3 pharmDx Interpretation Manual. The ongoing project aims to use an automated histological analysis tool to score immune checkpoint protein profiles in cancerous tissues and identify regions of tumour cells.
Acknowledgements
Thank you to Dr. Rithwik Ramachandran, Western University's USRI program, LAWSON-IRF grant and graduate students from Ramachandran lab for their support.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License
Document Type
Poster
Computer-Assisted Scoring of PD-L1 in Pancreatitis and Pancreatic Ductal Adenocarcinoma
My research project aims to examine the expression landscape of the immune checkpoint protein, PD-L1, in pancreatic ductal adenocarcinoma (PDAC) and pancreatitis using automated image analysis tools such as QuPath. Immunohistochemical analysis and confocal microscopy are utilized to identify unique expression patterns, including membrane staining and necrotic areas, compared to those identified in the PD-L1 IHC 22C3 pharmDx Interpretation Manual. The ongoing project aims to use an automated histological analysis tool to score immune checkpoint protein profiles in cancerous tissues and identify regions of tumour cells.