Faculty

Biochemistry

Supervisor Name

Dr. Chris Brandl

Keywords

stop codon readthrough, yeast, translation termination

Description

Stop codon readthrough occurs via genetic and epigenetic mechanisms, resulting in a longer polypeptide chain at the C-terminus. Although these readthroughs may seem like an error made by translational mechanisms, evidence from yeast suggests that stop codon readthrough has an impact on various cellular processes. Readthrough has the potential to create genetic diversity, similar to RNA splicing, mRNA editing, and protein modification. The diversity created by translational readthrough may result in a beneficial change in phenotype, and thus have a role in evolution and adaptability. Translational readthrough is observed in organisms such as yeast and E. coli but may also occur in more complex organisms such as humans. Because readthrough has the potential to cause phenotypical change, errors in the process may contribute to disease.

Acknowledgements

Dr. Chris Brandl

Julie Genereaux

Irina Petrovic

NSERC

Western University & USRI Program

Creative Commons License

Creative Commons Attribution-Noncommercial 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License

Document Type

Event

Included in

Biochemistry Commons

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Investigating stop codon readthrough in Saccharomyces cerevisiae

Stop codon readthrough occurs via genetic and epigenetic mechanisms, resulting in a longer polypeptide chain at the C-terminus. Although these readthroughs may seem like an error made by translational mechanisms, evidence from yeast suggests that stop codon readthrough has an impact on various cellular processes. Readthrough has the potential to create genetic diversity, similar to RNA splicing, mRNA editing, and protein modification. The diversity created by translational readthrough may result in a beneficial change in phenotype, and thus have a role in evolution and adaptability. Translational readthrough is observed in organisms such as yeast and E. coli but may also occur in more complex organisms such as humans. Because readthrough has the potential to cause phenotypical change, errors in the process may contribute to disease.