The infant intestinal microbiota in allergy

Authors

Lesleyann Hawthorn, University of Toronto
Gregor Reid, University of Toronto

Document Type

Article

Publication Date

1-1-1990

Journal

Journal of Biomedical Materials Research

Volume

24

Issue

10

First Page

1325

Last Page

1332

URL with Digital Object Identifier

10.1002/jbm.820241005

Abstract

Uropathogenic adhesion was measured using a range of polymer materials with differing surface tension properties. Experiments were carried out in the presence of phosphate buffered saline (controls), Tamm Horsfall protein (THP), and human urine with quantitation by image analysis. The results showed that THP did not bind to the polymer materials and therefore did not act as a receptor surface for type 1 fimbriated Escherichia coli. However, the THP did interfere with adhesion by binding directly to these organisms as well as to Pseudomonas aeruginosa, Staphylococcus epidermidis, and, to a lesser extent, Proteus mirabilis. Incubation of the uropathogens in THP and urine resulted in altered adhesion profiles to polymer surfaces, with no single trend apparent. The results emphasize that fluid components, particularly proteins, and substratum surface tension influence bacterial adhesion to biomaterials. Copyright © 1990 John Wiley & Sons, Inc.