Author

Olga Sazonova

Date of Award

2010

Degree Type

Thesis

Degree Name

Master of Science

Program

Physiology

Supervisor

Dr. Graham Wagner

Abstract

STC-l is expressed in kidney collecting duct cells and targeted to most other nephron segments. However, its function and regulation are both unclear. The present study explored the regulation of Stc-1 mRNA levels in vivo in rat models of dehydration and overhydration. Dehydration caused an upregulation in Stc-1 mRNA levels in kidney cortex and to a lesser extent the inner medulla. Antidiuretic hormone was identified as a potential mediator of gene induction during dehydration acting through the V2 receptor. In contrast overhydration did not produce changes in renal gene activity. Histological studies revealed no difference in STC-1 receptor distribution along the nephron indicative of similar targeting pathways irrespective of hydration states. Together the findings are suggestive of a novel role for renal STC-1 in the regulation of extracellular fluid volume, in particular during water deprivation.

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