Faculty
Faculty of Science
Supervisor Name
Sashko Damjanovski
Keywords
matrix metalloproteinase, mmp14, apoptosis sensitivity, BAX, FIH-1
Description
A member of the matrix metalloproteinase family (MMP14) plays a crucial role in extracellular matrix (ECM) remodeling, as it possesses characteristics that aid in cell proliferation and differentiation. MMP14 domains can bind to FIH-1, which is a protein that can prevent apoptosis in cells when attached to BAX in the mitochondria. This study will investigate the varying expressions of MMP14 on FIH-1 and BAX, and their effects on apoptotic sensitivity. Three cell lines with varying levels of MMP14 expression will be used: C3 (~11 fold), ΔCD (~100 fold), and C2 (~500 fold). With the overexpression of MM14 on the cell surface, cells are more susceptible to death, as FIH-1 has a higher affinity for the MMP14 domain as opposed to BAX. Caspase-Glo 3/7 Assay system will be used to assess apoptotic sensitivity after Staurosporine treatments. In essence, the significance of this research may aid in new therapeutic strategies for cancer treatments.
Acknowledgements
Thank you Dr. Damjanovski, the Western USRI program, and the Faculty of Science for their support.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License
Document Type
Event
Included in
Cancer Biology Commons, Cell Biology Commons, Other Cell and Developmental Biology Commons
The Role of Increased Matrix Metalloproteinase (MMP14) Expression on Apoptosis Sensitivity in Human Breast Cancer Cells
A member of the matrix metalloproteinase family (MMP14) plays a crucial role in extracellular matrix (ECM) remodeling, as it possesses characteristics that aid in cell proliferation and differentiation. MMP14 domains can bind to FIH-1, which is a protein that can prevent apoptosis in cells when attached to BAX in the mitochondria. This study will investigate the varying expressions of MMP14 on FIH-1 and BAX, and their effects on apoptotic sensitivity. Three cell lines with varying levels of MMP14 expression will be used: C3 (~11 fold), ΔCD (~100 fold), and C2 (~500 fold). With the overexpression of MM14 on the cell surface, cells are more susceptible to death, as FIH-1 has a higher affinity for the MMP14 domain as opposed to BAX. Caspase-Glo 3/7 Assay system will be used to assess apoptotic sensitivity after Staurosporine treatments. In essence, the significance of this research may aid in new therapeutic strategies for cancer treatments.