Faculty
Science
Supervisor Name
Vojislava Grbic
Keywords
RNAi, Pesticide, Genetics
Description
Tetranychus urticae are a species of pests that are very problematic in agricultural environments, as they cause lots of damage to a wide variety of crops. One step in figuring out a solution to this problem is through the use of RNA interference. After synchronizing the spider mites, they are treated with the dsRNA solution. The dsRNA is cleavage inside the mite, and the siRNA fragments then bind to a target gene in hopes of homologous sequence recognition leading to knockout/deregulation of the target gene. After treatment, the mites are observed for multiple variables, primarily fecundity and survival. If a dsRNA sequence is effective, compared to control mites, the dsRNA-treated mites should have significantly lower survival and fecundity.
Acknowledgements
Thank you to Dr. Vojislava Grbic and all the members in the Grbic lab, the Western USRI program, and the faculty of Science.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License
Document Type
Poster
Included in
RNA-Interference as a Topical Pesticide
Tetranychus urticae are a species of pests that are very problematic in agricultural environments, as they cause lots of damage to a wide variety of crops. One step in figuring out a solution to this problem is through the use of RNA interference. After synchronizing the spider mites, they are treated with the dsRNA solution. The dsRNA is cleavage inside the mite, and the siRNA fragments then bind to a target gene in hopes of homologous sequence recognition leading to knockout/deregulation of the target gene. After treatment, the mites are observed for multiple variables, primarily fecundity and survival. If a dsRNA sequence is effective, compared to control mites, the dsRNA-treated mites should have significantly lower survival and fecundity.