Faculty
Department of Pathology and Laboratory Medicine
Supervisor Name
Dr. Lee Cyn Ang & Dr. Qi Zhang
Keywords
Leukoencephalopathy, digital spatial profiling, microglia, neurodegeneration, CSF1R
Description
Adult-onset leukoencephalopathy with axonal spheroids (ALAS) is a group of hereditary, progressive, neurodegenerative disorders involving primarily the central nervous system white matter (WM). ALAS is characterized by patchy, asymmetrical myelin loss and axonal destruction in the WM, predominantly involving the frontoparietal regions. However, the asymmetrical and heterogenous involvement of different brain regions remains poorly characterized.
In this study, digital spatial profiling was performed to investigate the region-specific expressions of 60 proteins. Conventional immunohistochemistry methods was used validate intrepretation of probes. Using a high-plex and high-throughput method, we provide evidence of regional heterogeneity in ALAS, particularly involving key markers of microglia composition, glial cell subtype and autophagy.
Acknowledgements
I would like to thank the URSI program, my supervisors and lab members for their guidance and support:
- Dr. Lee Cyn Ang
- Dr. Qi Zhang
- Chelsey Zhao
- Carolyn Twible
- Rober Abdo
- Jane Ding
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Document Type
Poster
Included in
Medical Neurobiology Commons, Medical Pathology Commons, Nervous System Diseases Commons
Characterizing the Heterogeneity of Adult-Onset Leukoencephalopathy with Axonal Spheroids: A Digital Spatial Profiling Study
Adult-onset leukoencephalopathy with axonal spheroids (ALAS) is a group of hereditary, progressive, neurodegenerative disorders involving primarily the central nervous system white matter (WM). ALAS is characterized by patchy, asymmetrical myelin loss and axonal destruction in the WM, predominantly involving the frontoparietal regions. However, the asymmetrical and heterogenous involvement of different brain regions remains poorly characterized.
In this study, digital spatial profiling was performed to investigate the region-specific expressions of 60 proteins. Conventional immunohistochemistry methods was used validate intrepretation of probes. Using a high-plex and high-throughput method, we provide evidence of regional heterogeneity in ALAS, particularly involving key markers of microglia composition, glial cell subtype and autophagy.