Developing a Lap-On-Chip Sensor for Continuous Oxygen Monitoring in Microfluidics
Faculty
Faculty of Science
Supervisor Name
Tamie Poepping
Keywords
Microfluidics, Shear Stress, Luminescence Dynamic Quenching, Endothelial Cells, Biophysics
Description
A PDMS-based microfluidic device that is able to generate laminar and disturbed flow regimens at physiologically relevant wall shear magnitudes was previously developed. Considering the oxygen permeability of PDMS, it is ideal to investigate oxygen sensors that would provide a facile method for continuous monitoring of on-chip oxygen levels. In this project, a lab-on-chip oxygen sensor was developed that is based on the principle of dynamic quenching, to aid the microfluidic apparatus. This sensor would allow for monitoring and manipulation of oxygen in such a microfluidic setting, allowing for further understanding of the responses of endothelial cells to flow dynamics and their relevance to vascular health and disease.
Acknowledgements
I would like to express my gratitude to my supervisor, Dr. Tamie Poepping, who guided me throughout this project. I would also like to thank my lab co-workers who supported me and offered insights at every step of my project. Lastly, I would like to thank the organizers of the USRI fellowship and conference, for allowing me to partake in such an opportunity.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Document Type
Poster
Developing a Lap-On-Chip Sensor for Continuous Oxygen Monitoring in Microfluidics
A PDMS-based microfluidic device that is able to generate laminar and disturbed flow regimens at physiologically relevant wall shear magnitudes was previously developed. Considering the oxygen permeability of PDMS, it is ideal to investigate oxygen sensors that would provide a facile method for continuous monitoring of on-chip oxygen levels. In this project, a lab-on-chip oxygen sensor was developed that is based on the principle of dynamic quenching, to aid the microfluidic apparatus. This sensor would allow for monitoring and manipulation of oxygen in such a microfluidic setting, allowing for further understanding of the responses of endothelial cells to flow dynamics and their relevance to vascular health and disease.