Document Type
Article
Publication Date
2022
Journal
Journal of Neuroscience Methods
Volume
383
First Page
109737
URL with Digital Object Identifier
https://doi.org/10.1016/j.jneumeth.2022.109737
Abstract
Background: The small common marmoset (Callithrix jacchus) is an ideal nonhuman primate for awake fMRI in ultra-high field small animal MRI scanners. However, it can often be challenging in task-based fMRI experiments to provide a robust stimulus within the MRI environment while using hardware (an RF coil and restraint system) that is compatible with awake imaging. New Method: Here we present an RF coil and restraint system that permits unimpeded access to an awake marmoset’s head subsequent to immobilization, thereby permitting the setup of peripheral devices and stimuli proximal to the head.
Results: As an example application, an fMRI experiment probing whole-brain activation in response to marmoset vocalizations was conducted—this paradigm showed significant bilateral activation in the inferior colliculus, medial lateral geniculate nucleus, and auditory cortex.
Comparison with Existing Method(s): The coil performance was evaluated and compared to a previously published restraint system with integrated RF coil. The image and temporal SNR were improved by up to 58% and 27%, respectively, in the peripheral cortex and by 30% and 3% in the centre of the brain. The restraint-system topology limited head motion to less than 100 um of translation and 0.30° of rotation when measured over a 15-minute acquisition.
Conclusions: The proposed hardware solution provides a versatile approach to awake-marmoset imaging and, as demonstrated, can facilitate task-based auditory fMRI.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
Notes
Published version: https://doi.org/10.1016/j.jneumeth.2022.109737