Paediatrics Publications
Title
Suppression of the imprinted gene NNAT and X-Chromosome gene activation in isogenic human iPS cells
Document Type
Article
Publication Date
10-12-2011
Journal
PLoS ONE
Volume
6
Issue
10
URL with Digital Object Identifier
10.1371/journal.pone.0023436
Abstract
Genetic comparison between human embryonic stem cells and induced pluripotent stem cells has been hampered by genetic variation. To solve this problem, we have developed an isogenic system that allows direct comparison of induced pluripotent stem cells (hiPSCs) to their genetically matched human embryonic stem cells (hESCs). We show that hiPSCs have a highly similar transcriptome to hESCs. Global transcriptional profiling identified 102-154 genes (>2 fold) that showed a difference between isogenic hiPSCs and hESCs. A stringent analysis identified NNAT as a key imprinted gene that was dysregulated in hiPSCs. Furthermore, a disproportionate number of X-chromosome localized genes were over-expressed in female hiPSCs. Our results indicate that despite a remarkably close transcriptome to hESCs, isogenic hiPSCs have alterations in imprinting and regulation of X-chromosome genes. © 2011 Teichroeb et al.