β-Cell-specific pyruvate dehydrogenase deficiency impairs glucose-stimulated insulin secretion

Authors

Malathi Srinivasan, University at Buffalo, The State University of New York
Cheol S. Choi, Yale School of Medicine
Pushpankur Ghoshal, University at Buffalo, The State University of New York
Lioudmila Pliss, University at Buffalo, The State University of New York
Jignesh D. Pandya, University at Buffalo, The State University of New York
David Hill, Lawson Health Research InstituteFollow
Gary Cline, Yale School of Medicine
Mulchand S. Patel, University at Buffalo, The State University of New York

Document Type

Article

Publication Date

12-1-2010

Journal

American Journal of Physiology - Endocrinology and Metabolism

Volume

299

Issue

6

URL with Digital Object Identifier

10.1152/ajpendo.00339.2010

Abstract

Glucose-stimulated insulin secretion (GSIS) by β-cells requires the generation of ATP from oxidation of pyruvate as well as generation of coupling factors involving three different pyruvate cycling shuttles. The roles of several key enzymes involved in pyruvate cycling in β-cells have been documented using isolated islets and β-cell clonal lines. To investigate the role of the pyruvate dehydrogenase (PDH) complex (PDC) in GSIS, a murine model of β-cell-specific PDH deficiency (β-PDHKO) was created. Pancreatic insulin content was decreased in 1-day-old β-PDHKO male pups and adult male mice. The plasma insulin levels were decreased and blood glucose levels increased in β-PDHKO male mice from neonatal life onward. GSIS was reduced in isolated islets from β-PDHKO male mice with about 50% reduction in PDC activity. Impairment in a glucose tolerance test and in vivo insulin secretion during hyperglycemic clamp was evident in β-PDHKO adults. No change in the number or size of islets was found in pancreata from 4-wk-old β-PDHKO male mice. However, an increase in the mean size of individual β-cells in islets of these mice was observed. These findings show a key role of PDC in GSIS by pyruvate oxidation. This β-PDHKO mouse model represents the first mouse model in which a mitochondrial oxidative enzyme deletion by gene knockout has been employed to demonstrate an altered GSIS by β-cells. Copyright © 2010 the American Physiological Society.