Paediatrics Publications
Title
Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples
Document Type
Article
Publication Date
3-19-2021
Journal
STAR Protocols
Volume
2
Issue
1
URL with Digital Object Identifier
10.1016/j.xpro.2021.100311
Abstract
The examination of circulating pro-vascular progenitor cell frequency and function is integral in understanding aberrant blood vessel homeostasis in individuals with cardiometabolic disease. Here, we outline the characterization of progenitor cell subsets from peripheral blood using high aldehyde dehydrogenase (ALDH) activity, an intracellular detoxification enzyme previously associated with pro-vascular progenitor cell status. Using this protocol, cells can be examined by flow cytometry for ALDH activity and lineage restricted cell surface markers simultaneously. For complete details on the use and execution of this protocol, please refer to Terenzi et al. (2019) and Hess et al. (2019, 2020).