Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples

Authors

Daniella C. Terenzi, Keenan Research Centre for Biomedical Science
Ehab Bakbak, Keenan Research Centre for Biomedical Science
Justin Z. Trac, University of Toronto
Mohammad Al-Omran, University of Toronto
Adrian Quan, Keenan Research Centre for Biomedical Science
Hwee Teoh, University of Toronto
Subodh Verma, Keenan Research Centre for Biomedical Science
David A. Hess, University of TorontoFollow

Document Type

Article

Publication Date

3-19-2021

Journal

STAR Protocols

Volume

2

Issue

1

URL with Digital Object Identifier

10.1016/j.xpro.2021.100311

Abstract

The examination of circulating pro-vascular progenitor cell frequency and function is integral in understanding aberrant blood vessel homeostasis in individuals with cardiometabolic disease. Here, we outline the characterization of progenitor cell subsets from peripheral blood using high aldehyde dehydrogenase (ALDH) activity, an intracellular detoxification enzyme previously associated with pro-vascular progenitor cell status. Using this protocol, cells can be examined by flow cytometry for ALDH activity and lineage restricted cell surface markers simultaneously. For complete details on the use and execution of this protocol, please refer to Terenzi et al. (2019) and Hess et al. (2019, 2020).