Paediatrics Publications
Document Type
Article
Publication Date
11-1-2010
Journal
Journal of Magnetic Resonance Imaging
Volume
32
Issue
5
First Page
1068
Last Page
1075
URL with Digital Object Identifier
10.1002/jmri.22230
Abstract
Purpose: To demonstrate the feasibility and repeatability of cerebrovascular reactivity (CVR) imaging using a controlled CO2 challenge in mechanically ventilated juvenile pigs. Materials and Methods: Precise end-tidal partial pressure CO2 (PETCO2) control was achieved via a computer-controlled model-driven prospective end-tidal targeting (MPET) system integrated with mechanical ventilation using a custom-built secondary breathing circuit. Test-retest blood-oxygen level dependent (BOLD) CVR images were collected in nine juvenile pigs by quantifying the BOLD response to iso-oxic square-wave PETCO2 changes. For comparison, test-retest baseline arterial spin labeling (ASL) cerebral blood flow (CBF) images were collected. Repeatability was quantified using the intra-class correlation coefficient (ICC) and coefficient of variation (CV). Results: The repeatability of the PETCO2 (CV < 2%) step changes resulted in BOLD CVR ICC > 0.94 and CV < 6% for cortical brain regions, which was similar to ASL CBF repeatability (ICC > 0.96 and CV < 4%). Conclusion: This study demonstrates the feasibility and precision of CVR imaging with an MPET CO2 challenge in mechanically ventilated subjects using an animal model. Translation of this method into clinical imaging protocols may enable CVR imaging in young children with cerebrovascular disease who require general anesthesia. © 2010 Wiley-Liss, Inc.