Circulating Pro-Vascular Progenitor Cell Depletion During Type 2 Diabetes: Translational Insights Into the Prevention of Ischemic Complications in Diabetes

Authors

Daniella C. Terenzi, Keenan Research Centre for Biomedical Science
Mohammed Al-Omran, University of Toronto
Adrian Quan, Keenan Research Centre for Biomedical Science
Hwee Teoh, Keenan Research Centre for Biomedical Science
Subodh Verma, Keenan Research Centre for Biomedical Science
David A. Hess, Keenan Research Centre for Biomedical ScienceFollow

Document Type

Article

Publication Date

2-1-2019

Journal

JACC: Basic to Translational Science

Volume

4

Issue

1

First Page

98

Last Page

112

URL with Digital Object Identifier

10.1016/j.jacbts.2018.10.005

Abstract

Detection of vascular regenerative cell exhaustion is required to combat ischemic complications during type 2 diabetes mellitus (T2D). We used high aldehyde dehydrogenase (ALDH) activity and surface marker co-expression to develop a high-throughput flow cytometry–based assay to quantify circulating proangiogenic and proinflammatory cell content in the peripheral blood of individuals with T2D. Circulating proangiogenic monocytes expressing anti-inflammatory M2 markers were decreased in patients with T2D. Individuals with longer duration of T2D exhibited reduced frequencies of circulating proangiogenic ALDH hi CD34+ progenitor cells with primitive (CD133) and migratory (CXCR4) phenotypes. This approach consistently detected increased inflammatory cell burden and decreased provascular progenitor content in individuals with T2D.