An Rb-condensin II complex mediates long-range chromosome interactions and influences expression at divergently paired genes

Authors

Aren E. Marshall, Lawson Health Research Institute
Charles A. Ishak, Lawson Health Research Institute
Frederick A. Dick, Lawson Health Research InstituteFollow

Document Type

Article

Publication Date

1-1-2020

Journal

Molecular and Cellular Biology

Volume

40

Issue

2

URL with Digital Object Identifier

10.1128/MCB.00452-19

Abstract

Interphase chromosomes are organized into topologically associated domains in order to establish and maintain integrity of transcriptional programs that remain poorly understood. Here, we show that condensin II and TFIIIC are recruited to bidirectionally transcribed promoters by a mechanism that is dependent on the retinoblastoma (RB) protein. Long-range chromosome contacts are disrupted by loss of condensin II loading, which leads to altered expression at bidirectional gene pairs. This study demonstrates that mammalian condensin II functions to organize long-range chromosome contacts and regulate transcription at specific genes. In addition, RB dependence of condensin II suggests that widespread misregulation of chromosome contacts and transcriptional alterations are a consequence of RB mutation.