Probiotic interference of lactobacillus rhamnosus GR-1 and lactobacillus reuteri RC-14 with the opportunistic fungal pathogen candida albicans

Authors

Martine M.C. Velraeds, University of Groningen
Henny C. Van Der Mei, University of Groningen
Gregor Reid, The University of Western Ontario
Henk J. Busscher, University of Groningen

Document Type

Article

Publication Date

1-1-1996

Journal

Applied and Environmental Microbiology

Volume

62

Issue

6

First Page

1958

Last Page

1963

URL with Digital Object Identifier

10.1128/aem.62.6.1958-1963.1996

Abstract

In this study, 15 Lactobacillus isolates were found to produce biosurfactants in the mid-exponential and stationary growth phases. The stationary-phase biosurfactants from Lactobacillus casei subsp. rhamnosus 36 and ATCC 7469, Lactobacillus fermentum B54, and Lactobacillus acidophilus RC14 were investigated further to determine their capacity to inhibit the initial adhesion of uropathogenic Enterococcus faecalis 1131 to glass in a parallel-plate flow chamber. The initial deposition rate of E. faecalis to glass with an adsorbed biosurfactant layer from L. acidophilus RC14 or L. fermentum B54 was significantly decreased by approximately 70%, while the number of adhering enterococci after 4 h of adhesion was reduced by an average of 77%. The surface activity of the biosurfactants and their activity inhibiting the initial adhesion of E. faecalis 1131 were retained after dialysis (molecular weight cutoff, 6,000 to 8,000) and freeze-drying. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy revealed that the freeze-dried biosurfactants from L. acidophilus RC14 and L. fermentum B54 were richest in protein, while those from L. casei subsp. rhamnosus 36 and ATCC 7469 had relatively high polysaccharide and phosphate contents.