Physicochemical characteristics of two pairs of coagulase-negative staphylococcal isolates with different plasmid profiles

Authors

Martine M.C. Velraeds, University of Groningen
Henny C. Van der Mei, University of Groningen
Gregor Reid, University of Groningen
Henk J. Busscher, University of Groningen

Document Type

Article

Publication Date

5-1-1997

Journal

Urology

Volume

49

Issue

5

First Page

790

Last Page

794

URL with Digital Object Identifier

10.1016/S0090-4295(97)00065-4

Abstract

Objectives. The potency of the Lactobacillus acidophilus RC14 biosurfactant 'surlactin' to reduce the initial adhesion of Enterococcus faecalis 1131 was investigated on a hydrophilic and a hydrophobic substratum in a parallel-plate flow chamber, using phosphate-buffered saline and pooled human urine as a suspending fluid. Methods. A parallel-plate flow chamber with a glass or silicone rubber bottom plate was filled with different biosurfactant solutions of 0, 0.01, 0.1, or 1.0 mg/mL for overnight adsorption (18 hours). Subsequently, the adhesion of E. faecalis on thus prepared biosurfactant layers was observed in situ in phosphate-buffered saline or in urine by automated image analysis. Results. Adsorbed biosurfactant layers caused an important, dose-related inhibition of the initial deposition rate of E. faecalis and the number of adherent bacteria after 4 hours on both hydrophilic glass and hydrophobic silicone rubber, although this effect was stronger in buffer than in urine. For the experiments carried out in urine, the inhibitory effect of the biosurfactant layer was largest when silicone rubber was used rather than glass, whereas no influence of the substratum hydrophobicity on the inhibition of E. faecalis adhesion was noticed for experiments performed in buffer. Conclusions. The biosurfactant surlactin, as released by several Lactobacillus isolates, might open the way to the development of antiadhesive biologic coatings for catheter materials. It should be considered, however, that these results are preliminary and that the efficiency of the biosurfactant is probably affected not only by the hydrophobicity of the substratum and the suspending fluid, but also by the type of uropathogen involved.