Microbiology & Immunology Publications
Title
Evaluation of reuterin production in urogenital probiotic Lactobacillus reuteri RC-14
Document Type
Article
Publication Date
5-1-2006
Journal
Cancer Investigation
Volume
24
Issue
3
First Page
242
Last Page
251
URL with Digital Object Identifier
10.1080/07357900600629575
Abstract
Previous studies have shown that human PSP94 can inhibit the growth of prostate cancer cells both in vitro and in vivo. To further validate this potential and investigate the protein within a homologous setting, we examined the effects of rat PSP94 on the growth of the rat prostate adenocarcinoma cell line PAIII in vitro. To generate rat PSP94, we used both a plasmid-based expression system and a recombinant rat PSP molecule. Rat PSP was shown to inhibit the growth and survival of PAIII cells in a dose-dependent manner with >90 percent reductions in both observed. TUNEL and Annexin-V assays confirmed PAIII cell death to be via apoptosis. Copyright © Taylor & Francis Group, LLC.