Evaluation of reuterin production in urogenital probiotic Lactobacillus reuteri RC-14

Authors

Peter A. Cadieux, The University of Western Ontario
Sebastian A. Mikolajczak, The University of Western Ontario
Jonathan Reeves, Institut de Recherche en Immunologie et en Cancérologie de l’Université de Montréal
Craig Strathdee, The University of Western Ontario
Gregor Reid, The University of Western Ontario
Chandra J. Panchal, Institut de Recherche en Immunologie et en Cancérologie de l’Université de Montréal
Michael W. Clarke, The University of Western Ontario

Document Type

Article

Publication Date

5-1-2006

Journal

Cancer Investigation

Volume

24

Issue

3

First Page

242

Last Page

251

URL with Digital Object Identifier

10.1080/07357900600629575

Abstract

Previous studies have shown that human PSP94 can inhibit the growth of prostate cancer cells both in vitro and in vivo. To further validate this potential and investigate the protein within a homologous setting, we examined the effects of rat PSP94 on the growth of the rat prostate adenocarcinoma cell line PAIII in vitro. To generate rat PSP94, we used both a plasmid-based expression system and a recombinant rat PSP molecule. Rat PSP was shown to inhibit the growth and survival of PAIII cells in a dose-dependent manner with >90 percent reductions in both observed. TUNEL and Annexin-V assays confirmed PAIII cell death to be via apoptosis. Copyright © Taylor & Francis Group, LLC.