Effect of Lactobacillus rhamnosus GR-1 supernatant and fetal sex on lipopolysaccharide-induced cytokine and prostaglandin-regulating enzymes in human placental trophoblast cells: implications for treatment of bacterial vaginosis and prevention of preterm labor

Authors

Janice J. Liu, University of Washington, Seattle
Gregor Reid, Lawson Health Research Institute
Yonghou Jiang, University of Washington, Seattle
Mark S. Turner, Queensland University of Technology
Che Chung Tsai, University of Washington, Seattle

Document Type

Article

Publication Date

1-1-2007

Journal

Cellular Microbiology

Volume

9

Issue

1

First Page

120

Last Page

130

URL with Digital Object Identifier

10.1111/j.1462-5822.2006.00772.x

Abstract

Novel therapeutic approaches are needed to combat the rapid increase in HIV sexual transmission in women. The probiotic organism Lactobacillus reuteri RC-14 which safely colonizes the human vagina and prevents microbial infections, has been genetically modified to produce anti-HIV proteins which were capable of blocking the three main steps of HIV entry into human peripheral blood mononuclear cells. The HIV entry or fusion inhibitors were fused to the native expression and secretion signals of BspA, Mlp or Sep in L. reuteri RC-14 and the expression cassettes were stably inserted into the chromosome. L. reuteri RC-14 expressed the HIV inhibitors in cell wall-associated and secreted forms. L. reuteri RC-14 expressing CD4D1D2-antibody-like fusion proteins were able to bind single or dual tropic coreceptor-using HIV-1 primary isolates. This is the first study to show that a well-documented and proven human vaginal probiotic strain can express potent functional viral inhibitors, which may potentially lower the sexual transmission of HIV. © 2006 The Authors; Journal compilation © 2006 Blackwell Publishing Ltd.