Development and pilot evaluation of a novel probiotic mixture for the management of seasonal allergic rhinitis

Authors

Bita Baghchehsaraee, The University of Western Ontario
George Nakhla, The University of Western Ontario
Dimitre Karamanev, The University of Western Ontario
Argyrios Margaritis, The University of Western Ontario
Gregor Reid, The University of Western Ontario

Document Type

Article

Publication Date

8-1-2008

Journal

International Journal of Hydrogen Energy

Volume

33

Issue

15

First Page

4064

Last Page

4073

URL with Digital Object Identifier

10.1016/j.ijhydene.2008.05.069

Abstract

The effect of heat treatment at different temperatures on two types of inocula, activated sludge and anaerobically digested sludge, was investigated in batch cultures. Heat treatments were conducted at 65, 80 and 95 °C for 30 min. The untreated inocula produced less amount of hydrogen than the pretreated inocula, with lactic acid as the main metabolite. The maximum yields of 2.3 and 1.6 mol H /mol glucose were achieved for the 65 °C pretreated anaerobically digested and activated sludges, respectively. Approximately a 15% decrease in yield was observed with increasing pretreatment temperature from 65 to 95 °C concomitant with an increase in butyrate/acetate ratio from 1.5 to 2.4 for anaerobically digested sludge. The increase of pretreatment temperature of activated sludge to 95 °C suppressed the hydrogen production by lactic acid fermentation. DNA analysis of the microbial community showed that the elevated pretreatment temperatures reduced the species diversity. © 2008 International Association for Hydrogen Energy. 2