Electronic Thesis and Dissertation Repository

Degree

Master of Science

Program

Chemistry

Supervisor

Dr. Zhifeng Ding

Abstract

Scanning electrochemical microscopy offers a good insight into nickel surface analysis and human bladder cancer cell studies.

The surface microstructures are related with chemical reactivity by superimposing SECM, SEM and Tof-SIMS images together. Depth scans were exploited for studying chemical reactivity quantitatively by comparison with COMSOL simulation results. Simulation models are described.

Extracellular reactive oxygen species are detected by SECM. Quantitative results were demonstrated by simulation, and concentrations of H2O2 and O2 were acquired. The interaction between cancer cells and vitamin C was investigated. The switch-type behaviour of vitamin C on the PMA-induced ROS burst of T24 cells was evidenced: pro-oxidant in the first 30 min and anti-oxidant effect after 1 day.

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