Exploring Multimodal Cancer Cell Tracking Using Magnetic Particle Imaging and Akaluc BLI
Abstract
Magnetic Particle Imaging (MPI), a novel imaging technology, offers hotspot visualization and quantification of superparamagnetic iron oxide (SPIO) labelled cells in vivo. Bioluminescence imaging (BLI), with the sensitive reporter Akaluc, can provide complementary information on cell viability and proliferation. Here, we combined MPI, and Akaluc BLI for a more holistic picture of cancer cell fate in mice. Breast cancer cells labelled with Akaluc and the SPIO Synomag-D, were injected into the mammary fat pad (MFP) of mice and imaged on BLI and MPI for 2-weeks. Over this period, BLI signal increased due to tumour progression, while MPI signal decreased due to probe dilution in proliferating cells. Both modalities detected metastases, however, they were visualized in different locations. Overall, Akaluc BLI complemented MPI, providing sensitive detection of distant metastases, and longitudinal measures of cell viability. This multimodal approach should improve our understanding of metastasis, and aid development of novel therapeutics.