The Co-Delivery of Syngeneic Adipose-Derived Stromal Cells and Macrophages on Decellularized Adipose Tissue Bioscaffolds for In Vivo Soft Tissue Regeneration
Abstract
Decellularized adipose tissue (DAT) bioscaffolds are a promising platform for the delivery of pro-regenerative cell populations with the goal of promoting adipose tissue regeneration. The current study investigated the effects of seeding DAT bioscaffolds with syngeneic bone marrow-derived macrophages and/or adipose-derived stromal cells (ASCs) on in vivo soft tissue regeneration. Methods were established to derive the macrophages from MacGreen mice, which were dynamically seeded onto the DAT scaffolds alone or in combination with ASCs. Seeded and unseeded scaffolds were implanted subcutaneously into C57Bl/6 mice. At 2 and 4 weeks, cell infiltration, angiogenesis, and adipogenesis were analyzed through histology and immunohistochemistry. Substantial variability was observed, but higher blood vessel densities and greater CD31+ cell recruitment was observed in the implants that were greatly infiltrated with cells. A diverse, infiltrating macrophage population was identified in all implants, with quantitatively higher iNOS expression in the scaffolds that showed greater levels of cell infiltration.