Electronic Thesis and Dissertation Repository

Beta-cell β1 Integrin Deficiency During Second Transition Stage Of Fetal Pancreas Development On Islet Growth

Phyo Win, The University of Western Ontario

Abstract

β1 integrin has been shown to be important for pancreatic beta-cell growth and functioning. Congenital and postnatal studies have shown beta-cell mass reduction with β1 integrin knockout (β1KO). Using an inducible cre recombinase (CreERT) expressed from the mouse insulin promoter (MIP) crossed to a line in which Itgb1 (β1 integrin encoding gene) was flanked by loxP sites (MIP-CreERT+; β1itg designated as MIPβ1KO), we examined the in vivo temporal role of beta-cell β1 integrin effect on islet growth during the second transition stage of pancreas development.

Prenatal MIPβ1KO mice displayed decreased beta-cell area, mass, density and proliferation. In addition, a decrease in phosphorylated-ERK activity, islet vascularization and nascent endocrine cells in the ductal region was observed. However, normal levels of transcription factors needed for beta-cell development was seen. This study may suggest that β1 integrin plays an important role in prenatal beta-cell development during the second transition stage.