HcpE, a potential immuno-modulatory protein from Helicobacter pylori that is dependent on the Disulfide bond protein DsbHP

Author

Jeff Lester, The University of Western OntarioFollow

Degree

Master of Science

Program

Microbiology and Immunology

Supervisor

Dr. Carole Creuzenet

Abstract

H. pylori is a human gastric pathogen that colonizes ~ 50% of the world’s population. It can cause gastritis, gastric or duodenal ulcers and also gastric cancer. H. pylori produces Helicobacter cysteine rich protein HcpE, a secreted protein which may play a role in virulence. In this study we show that HcpE is secreted in the culture supernatant both as a soluble protein and in association with outer membrane vesicles, and may play a role in the modulation of H. pylori inflammatory responses. We identified that Dsb_HP is necessary for HcpE production and secretion in H. pylori, and demonstrated Dsb_HP has DiSulfide Bond (Dsb) forming activity on reduced lysozyme. Furthermore, we demonstrated that Dsb_HP has a DsbA-type of activity when expressed in E. coli, despite its similarity with DsbG, and that Dsb_HP is involved in maintaining redox homeostasis in H. pylori.

Recommended Citation

Lester, Jeff, "HcpE, a potential immuno-modulatory protein from Helicobacter pylori that is dependent on the Disulfide bond protein DsbHP" (2014). Electronic Thesis and Dissertation Repository. 2599.
https://ir.lib.uwo.ca/etd/2599