Electronic Thesis and Dissertation Repository

Degree

Master of Engineering Science

Program

Chemical and Biochemical Engineering

Supervisor

Dr. Wankei Wan

Abstract

In tissue engineering, the scaffold plays a critical role in guiding and supporting cells to function and grow optimally. The electrospun nanofibrous scaffold can serve as a near ideal substrate for tissue engineering because it has high surface area and the three-dimensional interconnected porous network can enhance cell attachment and proliferation. Core-shell nanofibrous scaffolds produced with coaxial electrospinning allow bioactive molecule encapsulation to improve cell adhesion, mediate and promote the proper signaling among the cells for their functioning and growth. In the current study, core-shell collagen nanofibers were fabricated via coaxial electrospinning with horizontal and vertical configurations. Core-shell nanofibers with optimum morphology and structure were stablized with Genipin, a natural crosslinking reagent extracted from the fruits of Gardenia jasminodies. The produced crosslinked core-shell collagen fibrous scaffolds have been proven to be cell compatible with improved structure stability and uniformity.

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