Electronic Thesis and Dissertation Repository


Master of Science




Dr. Michael Rieder

2nd Supervisor

Dr. Walter Siqueira

Joint Supervisor


The salivary proteome is recognized as a valuable source of potential oral and systemic disease biomarkers. Major efforts in salivary research have been dedicated to identify and characterize salivary proteins present in saliva using both classical biochemical methods and proteomics approaches in adults. Despite considerable research on the salivary proteome, little attention has been given to the changes in the salivary proteome occurring in children, specifically from 0-3 years of age. Through the use of anionic PAGE, SDS PAGE, HPLC and MS/MS, salivary protein profiles in children before, during and after dental eruption were compared with edentulous adult controls. We identified substantive qualitative and quantitative differences in the salivary proteome between children and adults, suggesting a greater emphasis is warranted in the study of the changes in the salivary proteome as a function of age and dental status.