Electronic Thesis and Dissertation Repository

Degree

Master of Science

Program

Biochemistry

Supervisor

Dr. Michael Rieder

2nd Supervisor

Dr. Walter Siqueira

Joint Supervisor

Abstract

The salivary proteome is recognized as a valuable source of potential oral and systemic disease biomarkers. Major efforts in salivary research have been dedicated to identify and characterize salivary proteins present in saliva using both classical biochemical methods and proteomics approaches in adults. Despite considerable research on the salivary proteome, little attention has been given to the changes in the salivary proteome occurring in children, specifically from 0-3 years of age. Through the use of anionic PAGE, SDS PAGE, HPLC and MS/MS, salivary protein profiles in children before, during and after dental eruption were compared with edentulous adult controls. We identified substantive qualitative and quantitative differences in the salivary proteome between children and adults, suggesting a greater emphasis is warranted in the study of the changes in the salivary proteome as a function of age and dental status.

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