Date of Award
2008
Degree Type
Thesis
Degree Name
Master of Science
Program
Biology
Supervisor
Dr. Susanne Kohalmi
Second Advisor
Dr. Mark Bernards
Third Advisor
Dr. Marc-André Lachance
Abstract
Six AROGENA TE DEHYDRA TASE (ADT) genes encode enzymes involved in phenylalanine synthesis in Arabidopsis thaliana. The tissue-specific RNA expression patterns of ADTs and their response to heat and cold stress were studied. Tissue-specific analyses indicated that ADTs are constitutively expressed at differing levels and that expression of particular ADTs is predominant in some tissues. Expression of all ADTs increased in response to cold treatment, either with a single (ADT1, ADT3, and ADT6), or bimodal (ADT2, ADT4, and ADT5) rise in expression. Expression returned to basal levels for all ADTs following a 24 h post-stress recovery. In response to heat stress only ADT2 and ADT4 transcript levels increased, whereas expression of all ADTs increased in response to a 24 h post-stress recovery. The results show that ADTs are differentially expressed in tissues and following the two stress treatments tested. These findings are consistent with the theory that different ADTs are needed for different phenylalanine requirements.
Recommended Citation
Hood, Rebecca Lynne, "RNA Expression of Six AROGENATE DEHYDRATASE Genes in Arabidopsis thaliana" (2008). Digitized Theses. 4705.
https://ir.lib.uwo.ca/digitizedtheses/4705