Date of Award


Degree Type


Degree Name

Master of Science




Dr. Earl Noble

Second Advisor

Dr. Jamie Melling

Third Advisor

Dr. Kevin Milne


The mechanisms controlling exercise-induced Hsp70 expression in skeletal muscle are not known. Hence, 12 week old male Sprague-Dawley rats were divided into sham (5 % DMSO) or PKA-inhibited (0.36 mg/kg H-89 in 5% DMSO) groups (n=4-8) and exercised for 60 min at 30 m/min on a 2% grade. Exercised animals were subsequently sacrificed at 10-15 min (PKA activity assay; phosphorylated ERK1∕2), 30 min (hsp70-l mRNA evaluated by RT-PCR) or 24 hr (Western blotting of Hsp70) post-exercise and the white vastus (WV), red vastus (RV), and soleus (SOL) were compared to appropriate non-exercised controls. Hsp70 protein increased following exercise in sham WV and RV but not SOL or PKA-inhibited (H-89) WV (p<0.05). Hsp70-l mRNA increased (p<0.05) in all muscles post-exercise regardless of H-89 treatment. Phosphorylated ERK1∕2 decreased following exercise (p<0.05) in PKA- inhibited WV. These data suggest that in skeletal muscle, unlike heart, post- transcriptional mechanisms control Hsp70 expression following exercise.



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