Date of Award
2006
Degree Type
Thesis
Degree Name
Master of Science
Program
Kinesiology
Supervisor
Dr. Earl Noble
Second Advisor
Dr. Jamie Melling
Third Advisor
Dr. Kevin Milne
Abstract
The mechanisms controlling exercise-induced Hsp70 expression in skeletal muscle are not known. Hence, 12 week old male Sprague-Dawley rats were divided into sham (5 % DMSO) or PKA-inhibited (0.36 mg/kg H-89 in 5% DMSO) groups (n=4-8) and exercised for 60 min at 30 m/min on a 2% grade. Exercised animals were subsequently sacrificed at 10-15 min (PKA activity assay; phosphorylated ERK1∕2), 30 min (hsp70-l mRNA evaluated by RT-PCR) or 24 hr (Western blotting of Hsp70) post-exercise and the white vastus (WV), red vastus (RV), and soleus (SOL) were compared to appropriate non-exercised controls. Hsp70 protein increased following exercise in sham WV and RV but not SOL or PKA-inhibited (H-89) WV (p<0.05). Hsp70-l mRNA increased (p<0.05) in all muscles post-exercise regardless of H-89 treatment. Phosphorylated ERK1∕2 decreased following exercise (p<0.05) in PKA- inhibited WV. These data suggest that in skeletal muscle, unlike heart, post- transcriptional mechanisms control Hsp70 expression following exercise.
Recommended Citation
Krause, Matthew P., "A role for PKA in exercise-induced Hsp70 expression in skeletal muscle" (2006). Digitized Theses. 4650.
https://ir.lib.uwo.ca/digitizedtheses/4650