Date of Award
2010
Degree Type
Thesis
Supervisor
Dr. Graham Wagner
Second Advisor
Dr. John Ciriello
Abstract
Staiiniocalcin-I (STC-1) is made by kidney collecting duct cells for targeting of local nephron segments. Currently, we have a poor understanding of how renal STC-1 is regulated or its role in kidney function. The present study has addressed this issue by examining renal STC-1 πιRNA expression levels in different models of kidney function. Adult rats were subject to either unilateral nephrectomy, water deprivation, hypertonicity or hypovolemia. Renal STC-1 mRNA levels were then quantified using real-time reverse transcription polymerase chain reaction (qPCR). Water deprivation had time-dependent stimulatory effects on STC-1 mRNA expression in kidney cortex and inner medulla. Similarly, hypertonicity increased cortical mRNA levels; however, the inner medulla was unaffected. In contrast, hypovolemia increased mRNA levels in the inner medulla and suppressed them in the cortex. Collectively, the findings suggest a novel role for mammalian STC-1 in kidney function as it may aid in the regulation of extracellular fluid volume and tonicity.
Recommended Citation
Turner, Jeffrey K., "REGULATION OF RENAL STANNIOCALCIN-1 GENE EXPRESSION IN RODENT KIDNEY BY HYPERTONICITY AND HYPOVOLEMIA" (2010). Digitized Theses. 4601.
https://ir.lib.uwo.ca/digitizedtheses/4601