Date of Award

2006

Degree Type

Thesis

Degree Name

Master of Science

Program

Biochemistry

Supervisor

Dr. Gilles Lajoie

Second Advisor

Dr. Victor Han

Abstract

Preeclampsia (PE) is a pregnancy specific disease that affects 5-8% of all pregnancies worldwide, and is one of the leading causes of maternal and neonatal morbidity and mortality. While the cause of PE is still unknown, clinical evidence unequivocally points to placenta as the site of pathophysiology. It is widely accepted that impaired cellular invasion during placental development and a lack of proper development of the decidual spiral arteries account for the development of PE. Despite considerable research, the factor(s) that ultimately cause PE have not been fully characterized. Through the use of 2D SDS-PAGE and LC MS∕MS, control and PE placental samples were compared at the proteomic level. This study identifies 12 differentially expressed proteins in PE. Many of these proteins are functionally related and point to several biological pathways or processes involved in the pathology of PE, suggesting a potential starting point for further biomarker discovery.

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