Date of Award
2008
Degree Type
Thesis
Degree Name
Master of Science
Program
Microbiology and Immunology
Supervisor
Dr. Gregory Dekaban
Second Advisor
Dr. Arthur Brown
Third Advisor
Dr. Bosco Chan
Abstract
Administration of a monoclonal antibody to the CDI ld∕CD18 integrin results in significant functional recovery in rodent models of spinal cord injury (SCI). This treatment selectively delays the influx of monocyte-derived hematogenous macrophages (hM0), suggesting that hM0 exhibit neurodegenerative or neuroprotective effects depending upon the temporal microenvironment of the SCI lesion. hM0 and microglial macrophages (mM0) are indistinguishable; hence, their roles in response to SCI remain unclear. Using SCI lys-EGFP-ki mice that enable distinction of EGFP+ hM0 from EGFP^ mM0, we demonstrate that both populations peak in the lesion at 7d post-SCI. The ‘classical inflammatory’ monocytes∕hM0 and ‘non-classical resident’ monocytes∕hM0 respond to the acute (ld, 3d, 7d) and chronic (14d, 6wks) stages of SCI, respectively. We report the depletion of a possibly novel blood subset in response to SCI that expresses monocyte and dendritic cell markers. Our study provides new insights for the mechanism of dichotomous response of hM0 to SCI.
Recommended Citation
THAWER, SAKINA G., "THE LYS-EGFP-KI MOUSE MODEL REVEALS PREVIOUSLY UNIDENTIFIED POPULATIONS OF HEMATOGENOUS AND MICROGLIAL MACROPHAGES FOLLOWING SPINAL CORD INJURY" (2008). Digitized Theses. 4402.
https://ir.lib.uwo.ca/digitizedtheses/4402