Date of Award

2008

Degree Type

Thesis

Degree Name

Master of Science

Program

Microbiology and Immunology

Supervisor

Dr. Gregor Reid

Abstract

The aims of this thesis were to select probiotic bacteria with potential to confer anti-allergy effects, develop a food formulation to deliver the strains, then test the concept in humans. Lactobacillus rhamnosus GR-1 and Bifidobacterium adolescentis 7007-05 were selected and successfully incorporated into yogurt. A total of 95 subjects participated in three studies. Immunoglobulin, cytokine and chemokines proved to be poor markers for allergy. Subjects who consumed probiotic yogurt for two months during grass pollen season tended to have improvements in their symptoms and signs of allergy. This was not found during a similar study in the ragweed pollen season in 2007. In a randomized, placebo- controlled trial performed in Mwanza, Tanzania, Lactobacillus reuteri RC-14 and Lactobacillus rhamnosus GR-1 in capsule form were administered for 10 weeks to HIV-infected women. Treatment induced significant increases in CD4 ∞unts, but this was the only evidence for skewing towards a Th1 immune response.

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