Date of Award
2009
Degree Type
Thesis
Degree Name
Master of Science
Program
Medical Biophysics
Supervisor
Dr. Paula Foster
Abstract
Successful migration of adequate numbers of dendritic cells (DC) to draining lymph nodes is crucial for effective DC immunotherapy. Since currently, less than 3% of injected DC migrate to lymph nodes, improving DC migration is necessary. A non- invasive method is required to monitor DC migration in animal models. This work presents the first report of using Micron-sized Superparamagnetic Iron-Oxide Particles (MPIO) to label DC for detection by MRI and of tracking their migration in a mouse model in vivo.
A quantitative approach was used to measure the signal loss in MR images and the fluorescence detected by microscopy. Moreover, the fate of MPIO-labeled DC was followed longitudinally. Labeling DC with MPIO for cell tracking provides an excellent option for imaging small numbers of cells in vivo for important preclinical investigations of disease
Recommended Citation
Rohani, Roja, "m VIVO TRACKING OF IRON-LABELED DENDRITIC CELLS IN MICE USING MAGNETIC RESONANCE IMAGING" (2009). Digitized Theses. 4314.
https://ir.lib.uwo.ca/digitizedtheses/4314