Date of Award
Master of Engineering Science
Dr. Wankei Wan.
Tissue engineering deals with creating tissues, using patient-derived cells, in order to restore, maintain or improve existing tissue function. In traditional tissue engineering, tissues are grown in-vitro prior to insertion in the body, and thus a scaffold is needed to support and guide the cells during growth. Nanofibrous scaffolds are considered promising since they mimic the extracellular matrix, have a high surface area-to-volume ratio, and possess excellent porosity and pore interconnectivity. In this research collagen type I nanofibers were fabricated using electrospinning. Collagen nanofibers were unstable in aqueous environments, and thus crosslinking was required. Glutaraldehyde, which is cytotoxic to cells, is currently the chemical crosslinking agent used by most research groups to stabilize collagen nanofibers. In this thesis, a novel approach, using genipin (a natural crosslinking agent), was introduced to crosslink the electrospun collagen nanofibers. Genipin has been proven to be significantly less cytotoxic compared to glutaraldehyde.
Mekhail, Mina, "Genipin-crosslinked electrospun collagen nanofibers" (2009). Digitized Theses. 4146.