Date of Award
2010
Degree Type
Thesis
Degree Name
Master of Science
Program
Physiology and Pharmacology
Supervisor
Dr. Frank Beier
Second Advisor
Dr. David Hess
Third Advisor
Dr. Chris Pin
Abstract
Osteoarthritis is a multifactorial disease that is characterized by cartilage degeneration. Manipulation of cells to form cartilage has been considered as a potential form of therapy, but currently there are no feasible molecular candidates. Recently this lab has identified four pharmacological compounds that could promote cartilage formation. We hypothesized that combinations of manuymycin A, PP2, Y27632, and cGMP would promote chondrogenesis more efficiently than individual compounds. Through fluorescent cytoskeleton imaging, toluidine blue staining, and real-time PCR, this study demonstrated that Manumycin A and cGMP had no effect on most tested parameters in ATDC5 cells. However, Y27632 caused significant changes in gene expression and PP2 negative affected glycosaminoglycan synthesis of ATDC5 chondrogenesis. Making them unsuitable for the intended promotion of articular cartilage generation. In summary, this study was not able to identify a compound, or combination of compounds that was able to successfully promote chondrogenesis without undesired effects.
Recommended Citation
Ladhani, Sadia F., "MOLECULAR APPROACH TO REGULATION OF CHONDROGENESIS OF ATDC5 CELLS" (2010). Digitized Theses. 4131.
https://ir.lib.uwo.ca/digitizedtheses/4131