Date of Award
2009
Degree Type
Thesis
Degree Name
Master of Science
Program
Biochemistry
Supervisor
Dr. Susan Meakin
Second Advisor
Dr. Eric Ball
Third Advisor
Dr. Shawn Li
Abstract
We believe that ShcC contributes to dephosphorylation of NMD A receptors. ShcC7' mice have increased levels of neuronal NR2B tyrosine phosphorylation and increased hippocampal long term potentiation. Our data demonstrates this increased phosphorylation is of tyrosine 1472 of NR2B indicating prolonged activity of the NMDA receptor. The data presented indicates that the ShcC SH2 domain does have the ability to interact with at least four tyrosine phosphorylated proteins and I have also identified at least three novel binding partners of ShcC: NKAIN2, Ubiquitin thiolesterase, and a protein similar to Glycoside hydrolase family 2 TIM barrel. Finally, it has been conclusively demonstrated that ShcC interacts with the protein tyrosine phosphatase STEP, and this interaction may utilize either the ShcC PTB or SH2 domain. ShcC may regulate the activity of STEP, and thus the phosphorylation of the NMDA receptor.
Recommended Citation
Grant, Robert Ian, "The Identification of Novel Binding Partners of the Neuronal Adaptor Protein ShcC" (2009). Digitized Theses. 3960.
https://ir.lib.uwo.ca/digitizedtheses/3960