Date of Award

2011

Degree Type

Thesis

Degree Name

Master of Science

Program

Anatomy and Cell Biology

Supervisor

Dr. Trevor Shepherd

Second Advisor

Dr. Gabriel DiMattia

Abstract

Current limitations regarding the treatment of metastatic epithelial ovarian cancer (EOC) are attributed to our poor overall understanding of its progression due to the limited number of appropriate model systems. To this end, I have characterized EOC tumour growth and angiogenesis using the innovative chick chorioallantoic membrane (CAM) model system. Bone morphogenetic protein (BMP) signaling has been implicated in multiple processes of EOC metastasis, although its role in tumour angiogenesis has never been assessed. I found an inverse correlation between the level of BMP signaling in mouse EOC cells and their abilities to induce angiogenesis both in vitro using HUVEC tube formation assays and in vivo using a chick CAM angiogenesis collagen onplant assay. These results support the further implementation of the chick CAM as an important tool to study EOC metastasis. Additionally, the putative anti-angiogenic role of BMP4 signaling from my work highlights potential future implications for this pathway in prognostics and treatment of EOC.

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.