Date of Award
2011
Degree Type
Thesis
Degree Name
Master of Science
Program
Chemistry
Supervisor
Dr. Ken Yeung
Abstract
Mass spectrometry (MS) has become the foremost detection method in protein analysis. In order to realize the sensitivity levels mass spectrometers are capable of, analytes must be purified from other components in the sample. This has become especially important with the trend toward miniaturization and development of microfluidic and lab-on-a-chip devices. In this thesis two sample preparation techniques for application in studying phosphorylated peptides were investigated using capillary electrophoresis. The first technique investigated a novel approach of integrating sample purification and preconcentration with the coupling of selective sample injection, based on the isoelectric point of the analytes, and enrichment using a pH junction. The integration of the two methods was successful. The second technique investigated miniaturization of a methyl esterification reaction. This reaction is commonly used when studying phosphorylated peptides to reduce the non-specific binding of acidic peptides during affinity chromatography. The reaction conditions were optimized and performed inside a capillary.
Recommended Citation
St.Pierre, Ashley, "Development of Capillary Electrophoresis Techniques for Protein Sample Preparation" (2011). Digitized Theses. 3381.
https://ir.lib.uwo.ca/digitizedtheses/3381