Date of Award

1982

Degree Type

Dissertation

Degree Name

Doctor of Philosophy

Abstract

Poxviruses are the most complex eukaryotic viral agents when considered in terms of size, morphology, and replicative strategy. Much of the life cycle of the prototypic member of this family, vaccinia virus, has been studied using metabolic inhibitors and, more recently, conditional-lethal temperature-sensitive (ts) mutants. In this study, biochemical, electron microscopic, and genetic analysis utilizing these tools has lead to a greater understanding of the roles, first, of post-translational cleavage (PTC) and envelope assembly and, secondly, of host DNA-dependent RNA polymerase II (polymerase II) and ongoing host gene expression in vaccinia virus biogenesis.

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