Non-thermal atmospheric pressure plasma-conditioned root dentin promotes attraction and attachment of primary human dental pulp stem cells in real-time Ex Vivo

Authors

Yeon Jee Yoo, Seoul National University School of Dentistry
Min Ji Kang, Seoul National University School of Dentistry
Hiran Perinpanayagam, Schulich School of Medicine & DentistryFollow
Joo Cheol Park, Seoul National University School of Dentistry
Seung Ho Baek, Seoul National University School of Dentistry
Kee Yeon Kum, Seoul National University School of Dentistry

Document Type

Article

Publication Date

8-1-2021

Journal

Applied Sciences (Switzerland)

Volume

11

Issue

15

URL with Digital Object Identifier

10.3390/app11156836

Abstract

This study investigated if non-thermal atmospheric pressure plasma (NTAPP) treatment of root dentin surfaces promotes human dental pulp stem cell (hDPSCs) adhesion. Freshly extracted human single-rooted teeth (n = 36) were decoronated and cut (first vertically, then horizontally) into root dentin slices (3 mm thick). Primary hDPSCs cultures were seeded onto slices randomly assigned to pretreatment groups (n = 9/group): NaOCl (1.5%), EDTA (17%) then NTAPP (Group I); NaOCl then NTAPP (Group II); NaOCl then EDTA (Group III); and NaOCl alone (Group IV). Cell viability and proliferation were measured using MTT assay with log-linear statistical analysis. Cell attachment and spreading morphologies on dentin slices (n = 3/group) were examined through scanning electron microscopy. Early cell adhesion events and subcellular activities were observed in real time by live-cell imaging through holotomographic microscopy. Cell viability and proliferation were significantly higher on NTAPP-treated dentin (p < 0.05), without interactions with EDTA (p > 0.05). The attachment, spreading, extensions and multiple layers of hDPSCs were heightened on NTAPP-treated dentin. Cell adhesion, spreading, and dentinal tubule penetration were hastened on NTAPP-treated dentin surfaces in real-time, with elevated subcellular activities and intracellular lipid droplet formation. NTAPP-treated root dentin surfaces support enhanced cellular responses, potentially promoting pulp-dentin regeneration.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.

Citation of this paper:

Yoo Y-J, Kang M-J, Perinpanayagam H, Park J-C, Baek S-H, Kum K-Y. Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo. Applied Sciences. 2021; 11(15):6836. https://doi.org/10.3390/app11156836