Controlling Differentiation of Adult Stem Cells Via Cell-Derived Nanoparticles: Implications in Bone Repair

Authors

Shruthi Polla Ravi, Western University
Yasmeen Shamiya, Western UniversityFollow
Aishik Chakraborty, Western UmiversityFollow
Ali Coyle, Western University
Alap A. Zahid, Western UniversityFollow
Jin Wang
Michael Boutilier, Western UniversityFollow
Emmanuel Ho
Arghya Paul, Western UniversityFollow

Document Type

Article

Publication Date

12-6-2022

Journal

ACS Applied Nano Material

Volume

12

First Page

17468

Last Page

17475

URL with Digital Object Identifier

https://doi.org/10.1021/acsanm.2c03657

Notes

Current nanostructured biomaterials-based drug delivery vehicles for bone regeneration applications often show suboptimal cellular uptake and inferior drug loading. To overcome these challenges, we have developed a biomimetic cell-derived nanoparticle (CDN) loaded with the Food and Drug Administration-approved small-molecule therapeutic Dexamethasone, to induce osteogenic differentiation in human adipose-derived stem cells. The drug-loaded CDNs were cytocompatible, maintained hydrodynamic stability with uniform spherical shape and size, and exhibited high-percentage drug loading along with rapid cellular uptake and stem cell differentiation. These results demonstrate for the first time the preparation of Dexamethasone-loaded CDNs capable of directing stem cell fate for advanced bone regeneration applications.