Modification of Polymer Networks with Bone Sialoprotein Promotes Cell Attachment and Spreading

Authors

Wailen D. Chan, The University of Western Ontario
Harvey A. Goldberg, The University of Western Ontario
Graeme K. Hunter, The University of Western Ontario
S. J. Dixon, The University of Western Ontario
Amin S. Rizkalla, The University of Western Ontario

Document Type

Article

Publication Date

9-1-2010

Journal

Journal of Biomedical Materials Research Part A

Volume

94A

Issue

3

First Page

945

Last Page

952

URL with Digital Object Identifier

http://dx.doi.org/10.1002/jbm.a.32715

Abstract

Biomaterials used for tissue engineering scaffolds act as temporary substrates, on which cells deposit newly synthesized extracellular matrix. In cartilage tissue engineering, polycaprolactone/poly(2-hydroxyethyl methacrylate) (PCL/pHEMA) polymer blends have been used as scaffold materials, but their use in osseous tissue engineering has been more limited. The objective of this study was to evaluate modification of PCL/pHEMA surfaces with bone sialoprotein (BSP), an extracellular matrix protein important in regulating osseous tissue formation. Modification of surfaces with BSP significantly enhanced osteoblastic cell attachment and spreading, without compromising proliferation. Thus, BSP-immobilization may be a useful strategy for optimizing scaffolds for skeletal tissue engineering.