Bone and Joint Institute
Thermoresponsive Self-Immolative Polyglyoxylamides
Document Type
Article
Publication Date
9-14-2020
Journal
Biomacromolecules
Volume
21
Issue
9
First Page
3817
Last Page
3825
URL with Digital Object Identifier
10.1021/acs.biomac.0c00899
Abstract
Thermoresponsive polymers with lower critical solution temperatures (LCSTs) are of significant interest for a wide range of applications from sensors to drug delivery vehicles. However, the most widely investigated LCST polymers have nondegradable backbones, limiting their applications in vivo or in the environment. Described here are thermoresponsive polymers based on a self-immolative polyglyoxylamide (PGAM) backbone. Poly(ethyl glyoxylate) was amidated with six different alkoxyalkyl amines to afford the corresponding PGAMs, and their cloud point temperatures (Tcps) were studied in water and buffer. Selected examples with promising thermoresponsive behavior were also studied in cell culture media, and their aggregation behavior was investigated using dynamic light scattering (DLS). The Tcps were effectively tuned by varying the pendent functional groups. These polymers depolymerized end-to-end following the cleavage of end-caps from their termini. The structures and aggregation behavior of the polymers influenced their rates of depolymerization, and, in turn, the depolymerization influenced their Tcp. Cell culture experiments indicated that the polymers exhibited low toxicity to C2C12 mouse myoblast cells. This interplay between LCST and depolymerization behavior, combined with low toxicity, makes this new class of polymers of particular interest for biomedical applications.
