Proteomic signature of the murine intervertebral disc

Authors

Matthew R. McCann, Western University
Priya Patel, Western University
Agya Frimpong, Western University
Yizhi Xiao, Western University
Walter L. Siqueira, Western University
Cheryle A. Séguin, Western University

Document Type

Article

Publication Date

2-17-2015

Journal

PLoS ONE

Volume

10

Issue

2

URL with Digital Object Identifier

10.1371/journal.pone.0117807

Abstract

© 2015 McCann et al. Low back pain is the most common musculoskeletal problem and the single most common cause of disability, often attributed to degeneration of the intervertebral disc. Lack of effective treatment is directly related to our limited understanding of the pathways responsible for maintaining disc health. While transcriptional analysis has permitted initial insights into the biology of the intervertebral disc, complete proteomic characterization is required. We therefore employed liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) protein/peptide separation and mass spectrometric analyses to characterize the protein content of intervertebral discs from skeletally mature wild-type mice. A total of 1360 proteins were identified and categorized using PANTHER. Identified proteins were primarily intracellular/plasma membrane (35%), organelle (30%), macromolecular complex (10%), extracellular region (9%). Molecular function categorization resulted in three distinct categories: catalytic activity (33%), binding (molecule interactions) (29%), and structural activity (13%). To validate our list, we confirmed the presence of 14 of 20 previously identified IVD-associated markers, including matrix proteins, transcriptional regulators, and secreted proteins. Immunohistochemical analysis confirmed distinct localization patterns of select protein with the intervertebral disc. Characterization of the protein composition of healthy intervertebral disc tissue is an important first step in identifying cellular processes and pathways disrupted during aging or disease progression.

Notes

© 2015 McCann et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

The version of record can be found at: McCann MR, Patel P, Frimpong A, Xiao Y,Siqueira WL, Séguin CA (2015) Proteomic Signatureof the Murine Intervertebral Disc. PLoS ONE 10(2):e0117807. doi:10.1371/journal.pone.011780

Data are availablethrough open-access at the Scholarship @ Westerninstitutional server (http://ir.lib.uwo.ca/physpharmpub/84/).

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.