Biology Publications
Document Type
Article
Publication Date
3-2019
Journal
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
Volume
229
First Page
40
Last Page
44
URL with Digital Object Identifier
https://doi.org/10.1016/j.cbpa.2018.11.016
Abstract
Dispersal is a central requirement of a successful sterile insect release programme, but field-released false codling moth (FCM) typically suffer from poor dispersal ability, especially at low ambient temperatures. Here we test the hypothesis that poor activity and dispersal in FCM is caused by delayed or perturbed recovery of ion and/or water homeostasis after chilling for handling and transport prior to field release. Hemolymph and flight muscle were collected from two treatment groups at three time points that targeted thermal conditions above and below the chill coma induction threshold of ~ 6 °C: 1) control moths kept at 25 °C, 2) moths exposed to 3 °C or 9 °C for 4 h, and 3) moths allowed to recover at 25 °C for 24 h after exposure to either 3 °C or 9 °C. We measured concentrations of Na+, K+ and Mg2+ in the hemolymph and muscle collected at each time point. Exposure to a chill-coma inducing temperature had little effect overall on ion balance in the hemolymph and flight muscle of false codling moth, but hemolymph [Na+] decreased from 10.4 ± 0.4 mM to 6.9 ± 0.7 mM as moths were chilled to 3 °C and then increased to 10.4 ± 0.9 mM after the 24 h recovery period. In the 9 °C cooling treatment, [K+] increased from 8.2 ± 0.5 mM during chilling to 14.1 ± 1.9 mM after the 24 h recovery period. No changes were seen in equilibrium potentials in either of the ions measured. Thus, we did not find evidence that water and ion homeostasis are lost by the moths in chill coma and conclude that reduced dispersal in field-released moths is not direct a consequence of the costs of re-establishment of homeostasis.
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Supplementary Material
Notes
This is an author-accepted manuscript. Final published version by Elsevier can be accessed at https://doi.org/10.1016/j.cbpa.2018.11.016