Biochemistry Theses and Dissertations

 

This collection contains theses and dissertations from the Department of Biochemistry, collected from the Scholarship@Western Electronic Thesis and Dissertation Repository

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Theses/Dissertations from 2024

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Human transfer RNA anticodon variants suppress pathogenic nonsense mutations, Aruun Beharry

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Identifying Shared Regions to Measure Change in Location of Spatial Transcriptomics, Siraj Elzagallaai

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Proteomic characterization of LIN28A-driven resistance to imatinib in Chronic Myeloid Leukemia, Owen F. J. Hovey

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Investigating the Dynamic Composition, Subcellular Localization, and Functions of the Human C-terminal to LisH Complex, Gabriel Onea

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Resolving the Longitudinal Triglyceride Phenotype of Heterozygous LPL and Apo A-V Deficiency, Shehan D. Perera

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Elucidating the temporal regulation of protein kinase CK2 by the peptidyl-prolyl isomerase Pin1, Scott E. Roffey

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Delivery and differentially phosphorylated AKT1 regulation in mammalian cells, Tarana Siddika

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Transfer RNA Missense Suppressors in Nature and Disease, Rasangi Tennakoon

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Kinome and Phosphor-proteome Dynamics of TCR/PD-1 Signalling and Strategies of Blocking PD-1 Signaling for Novel Immunotherapy, Shanshan Zhong

Theses/Dissertations from 2023

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Genetic Tools Towards A Synthetic Biology Approach For Whole Mitochondrial Genome Engineering, Ryan R. Cochrane

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Characterization of DNA Regulatory Elements of the Highly Abundant Secreted Protein 1 (HASP1) Promoter and Analysis of Transcriptomic Responses Under Phosphate Depletion in the Diatom Phaeodactylum tricornutum, Mahsa Farmanbar

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Multi-omics analysis of epithelial ovarian cancer metastasis, Mallory I. Frederick

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Functionalizing conjugative systems to deliver CRISPR nucleases for targeted bacterial killing, Thomas A. Hamilton

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Characterizing SaCas9 and SaCas9[D10E] tolerance to mismatches using Directed Evolution Using Fluorescence Activated Cell Sorting (DEUFACS), Olha Haydaychuk

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Selective Activation of Thrombin Activatable Fibrinolysis Inhibitor (TAFI) Attenuates Metastatic and Angiogenic Capabilities of Melanoma and Lung Carcinoma in vitro, Jacklyn Krizsan

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The inner workings of thrombin-activatable fibrinolysis inhibitor: a study of the TM-mediated activation of TAFI and inactivation of TAFIa, Haley D N Marier

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Advances in Phaeodactylum tricornutum nuclear engineering, Mark Pampuch

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Developing Regulated CRISPR Systems to Control Bacterial Microbiomes, Gregory M. Pellegrino

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State-of-the-art Approaches for Sequencing, Assembling and Annotating Naphthenic Acid Degrading Bacterial Metagenomes, Henry H. Say

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Mechanism of Permissive Cleavage Activity of TevCas12a, Alexa White

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Acetylation regulates Thioredoxin Reductase activity and oligomerization, DAVID E. WRIGHT

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Kinetic Analyses of SaCas9[D10E] in vitro, Claire Zhang

Theses/Dissertations from 2022

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The development of Sinorhizobium meliloti and Deinococcus radiodurans as chassis for synthetic biology applications, Stephanie L. Brumwell

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Applications of nanopore DNA sequencing for improved genome assembly, Daniel Giguere

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Dual Functions of Interstrand Crosslink Repair Nuclease SNM1A, Ryan Grainger

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Probing the genetic code with Leucine tRNA variants, Peter Anderson Hall

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Perseverance of protein homeostasis despite mistranslation, Farah Hasan

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Towards more complete metagenomic analyses through circularized genomes and conjugative elements, Benjamin R. Joris

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Dissecting the Molecular Basis of NRF2 Activity Modulation in the Oxidative Stress Response, Nadun Karunatilleke

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Chromatin regulation by RB-interacting proteins in cellular immune functions, Seung June Kim

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Identification and characterization of novel fumonisin-detoxifying enzymes, Michael C. Kovacevic

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Kinetic and structural influences of acetylation on ubiquitin processing, Rachel E. Lacoursiere

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Illuminating Transfer RNA Variants as Genetic Modifiers in Models of Human Disease, Jeremy T. Lant

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RHAMM as a biomarker and therapeutic target in triple-negative breast cancer, Britney Messam

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Characterization of disease-causing HARS mutations, Yi Qiu

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Global Regulation of miRNA and mRNA Homeostasis by Terminal Uridylyltransferases TENT3A/B, Pengcheng Zhang

Theses/Dissertations from 2021

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Mapping Ku70 Protein Interactions Using Proximity-Dependent Biotin Identification, Sanna Abbasi

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Applications of Genetic Testing for Endocrine and Metabolic Disorders, Amanda Berberich

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Implications and Applications of Transfer RNA Variants that Mistranslate the Genetic Code, Matthew D. Berg

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Biochemical and biophysical characterization of a novel fumonisin detoxifying enzyme from Aspergillus niger, Shane G. Butler

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Elucidating the Structural and Dynamical Properties of the Intrinsically Disordered Protein Nrf2 Using Molecular Dynamics Simulations, Megan Nicole Chang

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Investigating The Role Of PKC And Its Mechanisms In Regulation Of IGF-I Bioavailability In Fetal Growth Restriction, Allan W. Chen

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The Genetic Landscape of Neurodegenerative and Cerebrovascular Disease Phenotypes., Allison A. Dilliott

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New Perspectives on Phosphorylation State in the Parkin Ubiquitination Cascade, Karen Dunkerley

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The study of the structure and dynamics of parkin activation, Elaine Aisha Freeman

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Development of 3D Bioartificial Human Tissue Models of Periprosthetic Shoulder Joint Infection, Tony B. Huang

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tRNA Regulation in Humans: The cellular effect of a pathological HARS Y454S mutation, Rosan Kenana

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Genetic Approaches for the Study of Complex Human Diseases, Julieta Lazarte

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Uncovering the ubiquitin ligase activity and substrates of the human C-terminal to LisH (CTLH) complex, Matthew E.R. Maitland

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Characterizing the Role of TDG in FXR-dependent Signaling, Oladapo A. Onabote

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Functional Role of DREAM and DYRK1A in High-Grade Serous Ovarian Cancer Cell Dormancy, Pirunthan Perampalam

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Disruption of Insertion Sequence 200 (IS200) leads to a premature induction of the Cysteine regulon in Salmonella Typhimurium, Naomi-Jean Q. Scherba

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A Characterization of RGNEF Biophysical Properties and Interactome, Brooke E. Wile

Theses/Dissertations from 2020

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Nutrient Sensing Pathways Mediating IGFBP1 Phosphorylation in FGR, Shapnil Bhuiyan

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The Genetic Determinants of Complex Lipid and Lipoprotein Phenotypes, Jacqueline S. Dron

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Development of a Thermosensitive Endonuclease to Act as a Plasmid Kill-Switch, Christopher D. Leichthammer

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AWT1 is Constantly Expressed in Palmar Fascia Fibrosis and Promotes a Pro-Inflammatory Milieu, Johnny Luo

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Practical Applications and Future Directions of Genetic Code Expansion: Validation of Novel Akt1 Substrates and the Design of a Synthetic Auxotroph Strain of B. subtilis, McShane M. McKenna

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Deciphering the CK2-dependent phosphoproteome and its integration with regulatory PTM networks, Teresa Nunez de Villavicencio Diaz

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Generation of Conditional Ku70 Knockouts in Human Cell Lines Using CRISPR/Cas9 and Dual-Nuclease CRISPR/TevCas9, Gursimran Parmar

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Thyroxine-Dependent and -Independent Effects on Premature Aging and Myelination in ATRX Mutant Mice, Megan E. Rowland

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Development of the Model Marine Diatom Phaeodactylum tricornutum for Synthetic Biology Applications, Samuel S. Slattery

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The role of Heat Shock Protein 90 in the Keap1/Nrf2 Mediated Oxidative-Stress Response, Zheng Song

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Structural Study of the Complex Between DNA Polymerase Iota and Ub-PCNA, Harrison Taylor

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Quinone Reductase 2 Roles in Proteomic Regulation and Response to Treatment with Clinical Drugs, Matthew D. Walker

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Calcium signaling and pathogenesis of dysferlin C2 domains, Yuning Wang

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Determining Whether the Nature of the Amino Acid Substitution or the Extent of Mistranslation Affects the Impact of Mistranslating tRNAs in Saccharomyces cerevisiae., Yanrui Zhu

Theses/Dissertations from 2019

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Placental MicroRNA Expression in Pregnancies Complicated with Preeclampsia and Intrauterine Growth Restriction, Zain Awamleh

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Differentially activating the oncogenic kinase Akt1, Nileeka Balasuriya

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Regulation of RNA stability by terminal nucleotidyltransferases, Christina Z. Chung

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The Characterization of the Rat Bone Sialoprotein Knockout Phenotype, Benjamin Harvey

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Improving the genetic diagnosis of familial hypercholesterolemia, Michael Iacocca

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High Molecular-Weight Hyaluronan Prevents Basal Cell Carcinoma Via Promoting Apoptosis In Cancer-Initiating Adult Stem Cells, Violet Liu

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Applications of Phosphotyrosine Superbinding SH2 Domain Variants, Xuguang Liu

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Functional investigation of the role of the retinoblastoma protein in genome stability, Aren E. Marshall

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Structure and Function of Stomatin-like Protein 2, Safee Mian

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Nobiletin Corrects Intestinal Insulin Resistance and Lipid Metabolism in Ldlr-/- Mice Fed a High-fat Diet, Nadya Morrow

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Systematic identification of the lysine methylome using methyllysine binding domains, Wen Qin

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Investigating the Role of ATRX in Glutamatergic Hippocampal Neurons, Renee Tamming

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Structural and Functional Characterization of Deinococcal DNA Damage Response A (DdrA), Filip Todorovic

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Parkin Structural Perturbations in Oxidative Stress, An Tran

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Mass Spectrometry-Based Proteomics Analysis Of Bioactive Proteins In EMD That Modulate Adhesion Of Gingival Fibroblast To Improve Bio-Integration Of Dental Implants, David Zuanazzi Machado Jr

Theses/Dissertations from 2018

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Attenuation of Obesity, Hepatic Steatosis and Reversal of Atherosclerosis by the Flavonoids Naringenin and Nobiletin, Amy C. Burke

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Regulation of Beta-Catenin by the CTLH Complex, Christopher Chiasson

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Conformational Arrangements of UbcH7-Ubiquitin with OspG and Parkin, Tara E. C. Condos

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Chromatin organizer CTCF in brain development and behaviour, Adrienne Elbert

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Characterizing the Role of Thymine DNA Glycosylase in Transcriptional Regulation and Cancer In Vivo, Mohammad Haider Hassan

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Genetic determinants underlying rare diseases identified using next-generation sequencing technologies, Rosettia Ho

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The Role of Thymine-DNA Glycosylase In Transcriptional Regulation, Bart Kolendowski

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Metabolic and Expression Changes Associated with a Mouse Model of Intrauterine Growth Restriction (IUGR), Bethany N. Radford

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The Functional Characterization of the N-terminal Domains of TUT4, Lauren E. Seidl

Theses/Dissertations from 2017

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Autoinhibition and Activation of Parkin, Jacob D. Aguirre

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Characterizing the Cofactor Specificity of NQO2, Shahed Al Massri

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Role of High Molecular Weight Hyaluronan in Ultraviolet B Light-Induced Transformation, Katelyn Cousteils

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An Investigation of the CK2-dependent Phosphoproteome using Inhibitor Refractory CK2-alpha, Edward Cruise

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Identification of novel binding partners and substrates of histone H3K4 specific demethylase KDM5B/JARID1B, Qi Fang

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Effect on Pellicle Proteome upon Adsorption of Salivary Peptide on Hydroxyapatite and their effects on Enamel Demineralization, Rajesh Kr. Gupta

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Tti2 in PIKK Biosynthesis and Its Use in Identifying Missense Suppressor tRNAs, Kyle S. Hoffman

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The CDK-resistant pRB-E2F1 complex recruits chromatin-organizing proteins to repetitive DNA sequences, Charles A. Ishak

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Proteomic Characterization of Human Multipotent Stromal Cells Secreted Proteins with Therapeutic Potential for β-cell Regeneration, Miljan Kuljanin