Faculty

Medical Biophysics

Supervisor Name

Gediminas Cepinskas

Keywords

Sepsis. CORM3. Platelet adhesion. Human brain microvascular endothelial cells.

Description

Sepsis is characterized by the widespread inflammation of the body. Systemic inflammation activates and recruits inflammatory cells (e.g., leukocytes) and platelets to the affected organs.

During these inflammatory conditions, human brain microvascular endothelial cells (hBMEC) and platelets both upregulate adhesive molecules rendering platelets to adhere to hBMEC.

Although carbon monoxide is thought of as a toxic molecule to many, previous work shows its anti-inflammatory properties. Evidence has shown carbon monoxide-releasing molecules (e.g., CORM-3; that release small, non-toxic amounts of CO) can combat the effects of severe inflammation in several in vivo animal model.

In this current study, we are looking at whether administration of CORM-3 to hBMEC reduces platelet adhesion in vitro.

Acknowledgements

Special thanks to Dr. Eric Patterson, Dr. Gediminas Cepinskas, and Inga Cepinskas for helping me conduct this project through the summer.

Thanks go out to the Undergraduate Summer Research Internship program for funding me and project and allowing for this amazing experience.

Additional support; NSERC Discovery Grant (Dr. Cepinskas).

Document Type

Poster

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Effect of Carbon Monoxide Releasing Molecule 3 (CORM - 3) on Platelet Adhesion to Human Brain Microvascular Endothelial Cells

Sepsis is characterized by the widespread inflammation of the body. Systemic inflammation activates and recruits inflammatory cells (e.g., leukocytes) and platelets to the affected organs.

During these inflammatory conditions, human brain microvascular endothelial cells (hBMEC) and platelets both upregulate adhesive molecules rendering platelets to adhere to hBMEC.

Although carbon monoxide is thought of as a toxic molecule to many, previous work shows its anti-inflammatory properties. Evidence has shown carbon monoxide-releasing molecules (e.g., CORM-3; that release small, non-toxic amounts of CO) can combat the effects of severe inflammation in several in vivo animal model.

In this current study, we are looking at whether administration of CORM-3 to hBMEC reduces platelet adhesion in vitro.